The UC Irvine Chao Family Comprehensive Cancer Center's shared resources help foster innovative research into the causes, treatment and effects of cancer and allied diseases.
These units are supported by a National Cancer Institute Cancer Center Support Grant (P30CA062203) and therefore all publications related to work done by these cores must acknowledge grant and core, and must include a PMCID, as required by the NIH (see here and here for more information).
Suggested acknowledgement text:
“The authors wish to acknowledge the support of the Chao Family Comprehensive Cancer Center <SHARED RESOURCE NAME HERE> Shared Resource, supported by the National Cancer Institute of the National Institutes of Health under award number P30CA062203. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.”
The Biobehavioral Shared Resource (BBSR) provides a consultative service to assist researchers as they incorporate patient/participant reported outcomes into their projects. This support includes expertise on patient-reported outcome (PRO) instrument selection, PRO instrument development, and consideration for data collection assessment intervals and strategies to obtain valid and reliable PRO data. The BBSR has expanded service to include focus group design and conduct, offered in English and Spanish.
The Biostatistics Shared Resource (BSR) supports the conception, design, implementation, analysis and reporting of research conducted by members of the Chao Family Comprehensive Cancer Center. In addition, BSR faculty serve as liaisons to each of the cancer center’s Disease-Oriented Teams, keeping abreast of team developments and providing a first point of contact for assistance from the BSR with study design, data analysis, and new grant development.
The Experimental Tissue Shared Resource (ETR) provides basic, translational and clinical cancer center researchers access to, and analysis of, human and animal tissues. ETR leverages the technical and professional expertise of the Department of Pathology and Laboratory Medicine, offering four component services: Routine tissue histology/immunohistochemistry (IHC); tissue analysis, including laser capture microscopy (LCM); mouse pathology services; and genotyping services.
The Genomics High-Throughput Facility (GHTF) provides genome-wide analysis for clients interested in gene expression, regulation of gene expression, and genome sequence and variation. The primary forms of genome-wide analysis are the Affymetrix GeneChip, Illumina HiSeq 2500 next generation sequencing and PacBio RS real-time single molecule sequencing. Through campus shared resources, services for other technologies include NanoString and Ion Torrent PGM. The GHTF has expanded and currently provides services to numerous outside academic and non-profit institutions, as well as commercial biotech entities.
The In-Vivo Functional Onco-Imaging (IVFOI) shared resource supports basic and clinical cancer researchers by providing them with the necessary expertise, imaging instrumentation, and image analysis techniques. In addition to providing services with standard preclinical and clinical imaging systems available at most institutions, the IVFOI works to integrate molecular and structural imaging modalities to provide exclusive, first-of-its-kind multi-modality imaging technologies, providing for a better understanding of the fundamental biochemical nature of cancer and for development of new contrast agents and molecular pathway-specific imaging probes.
The Optical Biology Core (OBC) operates under the theme that technology enables discovery. Consequently, the goal of the OBC is to provide the best conventional microscopy platforms available and to develop and introduce novel optical methods that allow new discoveries to be made. OBC comprises three facilities on the UC Irvine campus: i) a self-use OBC facility equipped with fluorescence microscopes and image analysis software, ii) the Laser Microbeam and Medical Program (LAMMP), a collaborative facility dedicated to the use of lasers and other optics in Biology and Medicine, and iii) a flow cytometry facility in Hewitt Hall equipped with three multiparameter flowcytometers.
The Transgenic Mouse Facility (TMF) develops and provides services for making, breeding, genotyping, importing, and preserving genetically modified mice on a recharge basis. The TMF also offers advice and practical instruction in use of genetically modified mice in biological and biomedical research. In addition to supporting UCI investigators, approximately 70% of the services are provided to off campus investigators at academic and commercial institutions throughout the USA. The TMF provides standard services such as production of transgenic animals via pronuclear microinjection, development of ES cell derived gene-targeted modifications, embryo and sperm cryopreservation and reanimation, and genotyping. We also offer services in targeted transgenesis (at ROSA26 and Hprt), BAC transgenesis, tetraploid chimera production, development of new lines of ES cells, chromosome counting services, single blastomere injection, Southern analysis, and molecular cloning services to develop targeting constructs and BAC transgenes. The TMF has fully incorporated Cas9/gRNA based methodology into gene targeting methods and has used this to generate null mutations via NHEJ, generate point mutations via ssODN templated DNA repair and to generate floxed and knock-in alleles of mulitple loci via oligonucleotide and plasmid based targeting. A notable strength of the TMF is its ability to partner with PI's to develop new methodology for modification and analysis of the mouse. Since its inception in 1996, the TMF has generated hundreds of lines of genetically modified mice.
To find shared research core facilities provided by the other University of California Cancer Centers (UC Davis, UCLA, UCSD and UCSF), visit: please click here