The Transgenic Mouse Facility (TMF) facilitates the use of genetically modified mice in biological and biomedical research. An important goal of the TMF is to identify new technology and methodology that is likely to benefit UC Irvine investigators using the mouse in their research programs and to import and provide such technology to UC Irvine investigators. The TMF makes investigators aware of new and existing developments via the TMF website, in seminars and lectures, via the UC Irvine MouseUsers email list and by word of mouth.
Our primary goal is to offer cutting-edge services at the lowest cost while producing yields and outcomes that are as good as or better than similar facilities at other academic institutions. Our established track record documents our ability to help develop in vivo mammalian models of cancer with successful outcomes and to provide strains of mice that are critical to understanding the molecular basis of cancer. In a typical year we provide services to 30-40 different clients, on and off campus. Some services involve multi-year collaborations; (e.g., long-term breeding projects). Specialized equipment includes microinjection workstations, electrophoresis and Southern blotting equipment for high-throughput screening of embryonic stem cell clones, a controlled-rate freezer for cryopreservation of preimplantation stage embryos, and an IVF incubator. We have a proven track record in the manipulation of preimplantation stage embryos to produce genetically modified mice. During the past year we have introduced an exciting new service: inducible and reversible gene knockdown.
Director: Grant MacGregor, Ph.D.
Facility Manager: Tom Fielder
Finance Manager: Soo Lee
For more information about this resource or to request service, please visit The Transgenic Mouse Facility homepage.